|
KMID : 0545120120220101441
|
|
Journal of Microbiology and Biotechnology
2012 Volume.22 No. 10 p.1441 ~ p.1445
|
|
|
Cloning and Functional Verification of the Candida milleri HIS3 Gene Encoding Imidazoleglycerol Phosphate Dehydratase
|
|
Park Eun-Hee
Kwun Se-Young
Han Seung-Ah
Lee Jong-Sub
Kim Myoung-Dong
|
|
|
Abstract
|
|
|
|
The entire nucleotide sequence of the HIS3 gene encoding imidazoleglycerol phosphate dehydratase (IGPD) in yeast Candida milleri CBS 8195 was determined. Sequence analysis revealed an open-reading frame of C. milleri HIS3 that spans 678 bp, encodes 226 amino acids, and shares 80.5% amino acid identity to Torulaspora delbrueckii IGPD, followed by that to Saccharomyces cerevisiae (79.6%). The cloned HIS3 gene complemented a his3 mutation in S. cerevisiae, suggesting that it encodes a functional IGPD in C. milleri CBS 8195. A new auxotrophic marker is now available for acid-tolerant yeast C. milleri.
|
|
|
KEYWORD
|
|
|
Candida milleri, imidazoleglycerol phosphate dehydratase, HIS3
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|